Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQPFVLYNSEQRKKVEFVPRKEGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWKVRYIRNITDI--DDKIIKRANENGETIQQLTMRFIDAMNEDAANLGCLAPDEAPKATEYIDQMQNMIGNLVNKGAAYPASNGDVYFEVTKFEKYGRLSGRKLDDMQAGASERVDVEVEKKHPFDFVLW---KHAKENEPSWASPWGNGRPGWHIECSAMSTCCLGNHFDIHGGGSDLMFPHHENEIAQSEASTGEQYVNYWIHVGFINVDGEKMSKSLGNFFTIRDVMEK-FHPEVIRYFIVSSHYRSPVNFSDVALKEAKTS-------LTRFYHSFKAYQ--------QVYGQTTTETLDQSFVERFNNAMCDDFNTAEAMAVLFELNKELNRAVKEEQADQATVLYSTLRHLTNILGLVQHNVDDFLKSDIGQEAL-------ALSDAEIEDFIQQRVDAKKAKDFAKADSIRQSLLEQGVVLEDTRQGTVWRRAD 3SP1 Chain:B ((26-501)) -----LYNTRTKDFSELTNFE--NVKVYACGPTVYNYAHIGNFRTYIFGDLLIKTLRFLGYKVNYAMNITDIGH-----------GLTVYEISEFFTEAFFNDCRKLNIVYPDKVLVASKHIPIMIEVVKILEEKKITY-FSNGNVYFDTSCFKSYGEMAGI----------------KFKRNKTDFVLWFTNSKFKDQEMKWDSPWGFGYPSWHLECAAMNLEYFKDALDIHLGGVDHIGVHHINEIAIAECFLNKKWCDVFVHGEFLIMDYNKMS-----FITVKDLEDQNFSPLDFRYLCLTSHYRNQLKFSLDNLQASKIARENLINKLSYFYESLDPVDLNTLNKDLKNFGFSVEKEYYDSFVEK----ISFDLNVAQGLALLWEIIKSDNLS-----------FVSKLR-----LAFI---FDEIMSLNLREEILKNLQNHDVVIDENMKALIEERRIAKCEKNFKRADEIRDFFAKKGFVLVD---GTKVKRG-

General information: TITO was launched using: RESULT: Template: 3SP1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1966 -71367 -36.30 -175.78 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -36.30 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_3SP1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SP1-query.scw PDB file : Tito_Scwrl_3SP1.pdb: