Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRILTIGQKMPAWVLTGFEDYFKRIQPFVQTQVIKLPMAKRGKNDSEADILKYCQIEGESILNALKPNETLIALEVGGRELSTEKLADTMKQWMLEGNDVALAIGGPDGLSDQVRKAAAWHWSLSKLTMPHPLVRILLIEQLYRAMSINHNHPYHRA 5TWK Chain:A ((8-161)) VKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRGKN---ADIKRILDKEGEQMLAAAGKNR-IVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLIGGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHR-

General information: TITO was launched using: RESULT: Template: 5TWK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 693 -89869 -129.68 -583.56 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -129.68 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.619

(partial model without unconserved sides chains): PDB file : Tito_5TWK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TWK-query.scw PDB file : Tito_Scwrl_5TWK.pdb: