Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MKTFAPFFQVL-GISITLCTQAVFADEGISTQEADSLIKDDIASTQVLQEICPTFVGA-NKKLETNTQKIIAMYLSGYSNKSITFSALQNDSEYKTLLSEA-----RQAAKEMDHHEQHELCEEVVNYKD 5IK2 Chain:P ((1-134)) MATVQVDIVTPERKVFQGEADIVIARGVEGELGVMAGHIPLVTPLKTAPVRIKQGDKETLIAVSGGFLEVRPDKVNILADTAELPEEIAVEAAKKAKARHETILKRLDKTDKDYLRHKRALERAEVRLQVANSK-

General information: TITO was launched using: RESULT: Template: 5IK2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 475 24750 52.11 202.87 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain P : 0.69 3D Compatibility (PKB) : 52.11 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.035

(partial model without unconserved sides chains): PDB file : Tito_5IK2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IK2-query.scw PDB file : Tito_Scwrl_5IK2.pdb: