Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSAFKRF---------VQQSPSTVKDNVFAVVAY-------CPINNLANADLGY----------------------EWQYNASRNDSNTGNLNGVSYSAGPQLTASKEIAEKFPMYLQTLNLKL--PN-------GQQLTAENMPDQIKEQIK------SEIERQLAKGTPVPNFGENFVSSK-------ATLVNDWLKHDGSKVTEIDY--------QKFLNYVAANQALKTVVAFDAVGVNGNTAISGETN---LFGDSQNEYNNFTQ------------------WSWD--------HNSKTADGSGQDDTGLSWENYLNSNSSTANLLKDQLKMVNPIAYLNTTTDT-----APYWYIRHGVLDRDTSFAMQMILYY----------AVTNDPKVKDTNFKLPYLTGHAGNYDVQEAFKWINEKLNTTQ------------------------------ 4YB8 Chain:A ((1-464)) MNDLERLFNPSAIAVVGASKDPSKIGSQILRNLLSYGFKGKVYPINPTADELMGLKCYPKVSDVPDKVDVAVISVPSDKVLGVIDDCGKAGVKFAVVITSGFKEVGNEELEEELVRRAHSYGMRVLGPNIFGYLYAPARLNATFGPKDVLSGNVAFISQSGALGIALMGYTVVENIGISSIVSVGNKADLDDVDLLDFFDKDPNTGVIMIYLEGIAPGRGRMFIDVASRVSLRKPIIVIKAGRTEVGARAAASHTGSIAGSVAIYESAFKQSGILMAKSVEDAFDWTKALSWNPIPEGERLIVLTNGGGAGVQSTDTFADNGIYLSKPPESLIQEIKKFVPPFASFANPIDITGMAPDDWYYMGTLAALKNPDVDALTVLYCQTAVTTPIGVAKGIVDAIKEAGNSKPVTVGMVGGPEVAEAVSFLNKQRIAAYPTPERASSAMSALYAYARARSYVMKSLAVR

General information: TITO was launched using: RESULT: Template: 4YB8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1788 54324 30.38 168.71 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 30.38 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.087

(partial model without unconserved sides chains): PDB file : Tito_4YB8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YB8-query.scw PDB file : Tito_Scwrl_4YB8.pdb: