Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMILMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFWISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA 3FPF Chain:A ((2-265)) ---SCYIYWDKIKRIASRLEGMNYHFDEMDTSGVMPLLDEIEEIAH-----DSTIDFESAKHILDDAEMNHALSLI-RKFYVNLGMKLEMEKAQEVIESDSPWETLRS--FYFYPRYLELLKNEAALGRFRRGERAVFIGGGPLP----------------LTGILLSHVYGMRVNVVEIEPDIAELSRKVIEGLGVDGVNVITGDETVIDGLEFDVLMVAALAEP--------------KRRVFRNIHRYVDTETRIIYRTYTGMRAILYAPVSDDDITGFRRAGVVLPSGKVNNTSVLVFKCP----------------

General information: TITO was launched using: RESULT: Template: 3FPF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1331 -285050 -214.16 -1079.73 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -214.16 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.140

(partial model without unconserved sides chains): PDB file : Tito_3FPF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FPF-query.scw PDB file : Tito_Scwrl_3FPF.pdb: