Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINIHEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPKKEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHILDKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIK-EEHLEDKCRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGIS-------RQQGGATNAWINKY-------IFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFHQVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA 1KPH Chain:C ((11-283)) -----------------------------------------------------------------------------------------------------------------NVQAHYDLSDDFFRLFLDPTQTYSCAYFERDDMTLQEAQIAKIDLALGKLGLQPGMTLLDVGCGWGATMMRAVEKYDVNVVGLTLSKNQANHVQQLVANSENLRSK-RVLLAGWEQFD-EPVDRIVSIGAFEHFGHERYDAFFSLAHRLLPADGVMLLHTITGLHPKEIHERGLPMSFTFARFLKFIVTEIFPGGRLPSIPMVQECASANGFTVTRVQSLQPHYAKTLDLWSAALQANKGQAIALQSEEVYERYMKYLTGCAEMFRIGYIDVNQF---------------

General information: TITO was launched using: RESULT: Template: 1KPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1225 -74360 -60.70 -288.22 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.72 3D Compatibility (PKB) : -60.70 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_1KPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KPH-query.scw PDB file : Tito_Scwrl_1KPH.pdb: