Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEELTSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLHVVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSITAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVADSSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPEREGKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQMFIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKI-SRNQLYWTNMLLAILFAILAILISSGALGLSALASMKDAK-DMDLLMFLKAGFNFLPAVLVFIGISGFLFGWLPRIGK----LAYIYLAYSFALNYFGGILDLPEWFS---KTAVPSWVPRMPN----DSF----------------------------DLSVFLTLSCISLALFVLGSIGYRRRDLLEG 5DO7 Chain:B ((471-670)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYI--IIYGMPTYWL-----ANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQK--

General information: TITO was launched using: RESULT: Template: 5DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 441 -72890 -165.28 -458.42 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.46 3D Compatibility (PKB) : -165.28 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.206

(partial model without unconserved sides chains): PDB file : Tito_5DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DO7-query.scw PDB file : Tito_Scwrl_5DO7.pdb: