Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGIVSNDRGK-LKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFKFHDQSIGLAHNGNLINARSLRKEL-EENGAIFHSNSDTEILMHLIRRSE---------ETDFLNKLKSALNE-VKGGFAYLLMT-ETAMIAALDPNGFRPLAIGQM----KNGAYVIASETCALEVIGARFIRDVQPGEIVIINDEGIQIDCFTKDTQLS-ICSMEYIYFARPDSNIAGINVHTARKNMGRNLAKE-----SPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELREQGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKYPCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCMAYFNGDYPTPLYDYEEKYQESLKEKVSFF 1ECG Chain:B ((1-478)) -----------CGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFRLRKANGLVSDVF-EARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVN-SPYGITLAHNGNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFA-SELDNFRHYPLEADNIFAAIAATNRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLGFDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSARVNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRTFIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYLASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAENPDIQQFEC-SVFNGVYVTK--DVDQGYLDFL-------

General information: TITO was launched using: RESULT: Template: 1ECG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2793 8917 3.19 19.60 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : 3.19 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_1ECG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ECG-query.scw PDB file : Tito_Scwrl_1ECG.pdb: