Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYLTLPGDD--YELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPGTTPSYY-FVIDPDGYKIEVIRG 4MTS Chain:B ((1-125)) MRILHSMLRVADLEAALEFYTRALDMRLLRRRDYPEGRFTLAFVGYQDERAAAALELTHNWDRDGYTQGDGYGHLAIEVEDAAVTCARARALGYRVTREAGLMQHGRSVIAFLEDPDGYKVELIQ-

General information: TITO was launched using: RESULT: Template: 4MTS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 474 -17800 -37.55 -145.90 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -37.55 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.652

(partial model without unconserved sides chains): PDB file : Tito_4MTS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MTS-query.scw PDB file : Tito_Scwrl_4MTS.pdb: