Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------MKTKQLTIYAMLIALTVALSLTILI--PVPATNGFVTLCEAGIYTAAFLYGPTGGLVVGASS-GLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSST--RMWGLGLLLGSLV-------------MIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQY 5JI7 Chain:A ((40-238)) LQRRLKRLYPAVDEQETPLPRSWSPKDKFSYIGLSQNNLRVHYKGHGKTPKDAASVRATHPIPAACG-IYYFEVKIVSK----GRDGYMGIGLSAQGVNMNRLPGWDKH---SYGYHGDDGHSFCSSGTGQPYGPTFTTGDVIGCCVNLINNTCFYTKNGHSLGIAFTDLPPNLYPTVGLQTPGEVVDANFG---QHPFVFDIEDYMREW--

General information: TITO was launched using: RESULT: Template: 5JI7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 643 -35430 -55.10 -239.39 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -55.10 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_5JI7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JI7-query.scw PDB file : Tito_Scwrl_5JI7.pdb: