Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GPCVITIDGPYNPKYNFYPSHGRGVTGPHGQVVIGDFT-CTVG----------ATC-NQLTCNAPQPEYSILGVDSSKWGLSDTESDSD----------SDSNSKQAIMPHNRKQVAPVSHY 5HS5 Chain:A ((2-117)) -----TLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHCKDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKE-RLSKVRSSKDERKIYIYLNNDDISKFNALFEDVEQFLNILEHH

General information: TITO was launched using: RESULT: Template: 5HS5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 268 7098 26.48 75.51 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 26.48 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.092

(partial model without unconserved sides chains): PDB file : Tito_5HS5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HS5-query.scw PDB file : Tito_Scwrl_5HS5.pdb: