Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence FCVYYDGHLPATRVLLMYVRIGTTATITARGHEFEVEAKD----QNCKVILTNGKQAPD---WLAAEPY 5I4K Chain:A ((44-80)) ----------------------------VDPHEYDPLPANIQSAADADLIFYNGLNLETGNGWFD----

General information: TITO was launched using: RESULT: Template: 5I4K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 26 1535 59.04 51.17 target 2D structure prediction score : 0.87 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 59.04 2D Compatibility (Sec. Struct. Predict.) : 0.87 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.589

(partial model without unconserved sides chains): PDB file : Tito_5I4K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I4K-query.scw PDB file : Tito_Scwrl_5I4K.pdb: