Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WLFEGLGRDKAEELLQLPDTKVGSFMIRESETKKGFYSLSVRH---RQ---VKHYRIFRLPNNWYYISPRLTFQCLEDLVN 4LUE Chain:B ((72-176)) WFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRT----GFYISPRSTFSTLQELVD

General information: TITO was launched using: RESULT: Template: 4LUE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 231 -9383 -40.62 -132.15 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -40.62 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_4LUE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LUE-query.scw PDB file : Tito_Scwrl_4LUE.pdb: