Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYSKHMTRSQAEQLLKQEGKEGGFIVRDS-SKAGKYTVSVFAK----STGDPQGVIRHYVVCSTPQSQYYLAARNCLVND 2RMX Chain:A ((8-69)) WYHGHMSGGQAETLLQAKGEPWTFLVRESLSQPGDFVLSVLSDQPKAGPGSPLRVTHIKVMC------------------

General information: TITO was launched using: RESULT: Template: 2RMX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 189 -18020 -95.34 -316.14 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -95.34 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.735

(partial model without unconserved sides chains): PDB file : Tito_2RMX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RMX-query.scw PDB file : Tito_Scwrl_2RMX.pdb: