Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------MKKSSILF-------------IFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFLALFYMIPALYFLFR--IGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKFL--FLLGIASGLGFQMIEDI-------GYIR------------TDLPEGFDFT--ISRILERI--ISGIAS---------------HWTFSG----LAVVG---VYLL-----YRAY--KGQKV-GKKQGLIV 5I5H Chain:A ((246-572)) YPLSELRYRDMGTGQNVLLITVDGLNYSRFEKQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITALNQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNRWFSWVSFNGTNIDDSNQQAFARKY-SRAAGNVDDQINRVLNALRDSGKLDNTVVIITAGRGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTPASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDEKPQLSL

General information: TITO was launched using: RESULT: Template: 5I5H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1052 -48161 -45.78 -220.92 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -45.78 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.130

(partial model without unconserved sides chains): PDB file : Tito_5I5H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I5H-query.scw PDB file : Tito_Scwrl_5I5H.pdb: