Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFD-----DTPTEVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDP----EIP-----NSLEIAHKRGIKI------VWTIQKDSNAPHPNTTKITVKNAHK--TISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHNVNFFK 2IAF Chain:A ((7-144)) ---------------------SSHTVGPMLAANAFLQLLEQKNLFDKTQRVKVELYGSLALTGKGHGTDKAILNGLENKAPE--SMIPRMHEILDSNLLNLAGKKEIPFHEATDFLFLQKELL-PKHSNGMRFSAFDGNANLLIEQVYYSIGGGFIT----------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2IAF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 481 21391 44.47 192.71 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 44.47 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_2IAF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IAF-query.scw PDB file : Tito_Scwrl_2IAF.pdb: