Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFFPSEQEQIEKFEKDHVAQHYFEVLRTLI---SKKSVFAQQVGLKEVANYLGE-IFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVWTEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESASTDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIHSSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGL-ELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYPQGVSVLPTTAGTGPMHTVFDALEV--PMVAFGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE 4MMO Chain:B ((8-434)) ----------------------------LIEFLKKPSI--TGEGIDETANYLKETVEKLLGVKANLEKTKGHPVVYAEINVNA--KKTLLIYNHY--------------PFSAT-----------SDNKGTLMARLFAI-KHLLDKNELNVNVKLLYEG-----SVNLEDYIEKNTNKLK-ADSVIMEGAGLDPKGRPQIVLGVKGLLYVELVLDYGTKDLHSSNAPLVRNPCIDLAKIISTLVDMGGRVLIEGFYDDVRELTEEERELIKKY-DIDVEELKKALGFKELKYNEKEKIA--EALLTYPTCNVDGFECGYTGKGSKTIVPHRAFAKLDFRLVPNQDPYKVFELLKKHLQKAGFNGEILAHGF-EYPVRTSVNSTVVKAMIESAKKVYG-EPQVIPNSAGTQPMGLFVYKLGIRDAVSAI---------------IKIDDYYKAIKHTEEFLKLY-

General information: TITO was launched using: RESULT: Template: 4MMO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1801 19639 10.90 52.79 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : 10.90 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_4MMO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MMO-query.scw PDB file : Tito_Scwrl_4MMO.pdb: