Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSYRIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLAIYGKMEREGKKFGLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIAAEDLVLYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQQIKQNINQNFLTSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQLKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDKS 4UTG Chain:B ((115-159)) ------------------------------------------------------------------------------------------------------GLGSSSTLVVVMIKAFVELLNLPLDDYAIAQL--AYRIERVDCGLAG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4UTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 86 -22588 -262.65 -501.94 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -262.65 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.698

(partial model without unconserved sides chains): PDB file : Tito_4UTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UTG-query.scw PDB file : Tito_Scwrl_4UTG.pdb: