Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLN-SRGAISEELLAEFSN-YGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTEADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGLIGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVADLTVFLAGQ--DYLTGQVIAIDGGLSM 2PNF Chain:B ((3-247)) IKLQGKVSLVTGSTRGIGRAIAEKLASAGSTVIITGTSGERAKAVAEEIANKYGVKAHGVEMNLLSEESINKAFEEIYNLVDGIDILVNNAGITRDKLFLRMSLLDWEEVLKVNLTGTFLVTQNSLRKMIKQRWGRIVNISSVVGFTGNVGQVNYSTTKAGLIGFTKSLAKELAPRNVLVNAVAPGFIETDMTAVLSEEIKQKYKEQIPLGRFGSPEEVANVVLFLCSELASYITGEVIHVNGGM--

General information: TITO was launched using: RESULT: Template: 2PNF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1302 -21034 -16.16 -87.28 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -16.16 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_2PNF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PNF-query.scw PDB file : Tito_Scwrl_2PNF.pdb: