Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDVFLITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVKESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQSMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADY-PFKYLAGCGVAFKLACALLEEV----QVELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEETVGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEAKTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAVDIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNLDEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISKGEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFNIRGKNA---------VL----P--------EG--VPVLDFP-----GELPNLAASEAVVVKNIPEDITQLKTIFQEQ-HFSAVYFKNDIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKM------IQVFEELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE 5F56 Chain:A ((2-676)) ------SRPAHWLLAPPASRDALLATMREWQVSPPVAQVLCGRDLRTE-----LLALPLELTPN----PALREAARHIVAAVREGKRIRIHGDYDADGVSATATLVLGLRAIGANVHGFIPHRLNEGYGIHPDRVPEH-AA-AADLVVTVDCGVSNLDEVKSLLATGTEVVVTDHHAPGENFPEC-LVVHPHLTPDYDPDRHNLTGAGVAYHLLWAVYEELGRPEPRALLPLATLGTVADVAPLLGENRALVRAGLAEMARTELPGLRALMNEKRV--RQPTARDVAFILAPRINAAGRMGEADRALELLTTPSDHEAKSLAAYLEIRNQERRKIQDDMFAQALQLADPNDPALVLTHDDWHAGVMGIVASKLVETFNRPVYIVAQ----GKGSVRSTPGISAVQGLRESRDLLGRFGGHPGAAGFSLDPQNFGALRERIHGYVRQFPT---PVPAVRLDAPLPVAALTPELLSELSILEPFGEGNPRPLWHLRGPLT-DTRLVGKQGDVLQFRFG----GVKGMKYSERDD-----AAGERDVAAELALNEWKGRTSLELHAAALRPLAPLALAGTEEGLPTLPRLNPREAMTFLKTGAAAYAEQGVATYLRDNVPGLTLLDTNAPHPGGDLILYGLPPESALRRWLHEAQEQGGRVAFALGP---------KTLAELDAALTLAKLLPDS--------------HTEAAQEAAADAYRSWQWAHHYRVLNDAG-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5F56.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3528 -53570 -15.18 -84.36 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -15.18 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_5F56.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F56-query.scw PDB file : Tito_Scwrl_5F56.pdb: