Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLAEFYSKEGNMNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDTVKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDILPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVYRLKLSGTFVYLPEN--NMLGFIHPSERY--------AEPRLGQVLDARVIGFREVDRTLNLSLKPRSFEMLE---------NDAQMILTYLE-SNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQDQFGTELI 1YZ6 Chain:A ((8-144)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------YPEEGEFVVATVKRIHNYGAFLELD-EYPGKEAFMHISEVASTWVRNIRDYLKEGQKVVAKVIRVDPRKGHIDLSLRRVTQQQRKAKLQEFKRAQKAENLLKLAAEKLGKDFETAWREVWVPLEEEWGEVYAAFEDAA-------------------

General information: TITO was launched using: RESULT: Template: 1YZ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 440 12980 29.50 110.94 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 29.50 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_1YZ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YZ6-query.scw PDB file : Tito_Scwrl_1YZ6.pdb: