Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRLETMKVVAQEEIAPAIFELVLEGEMV-EAMRAGQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDT-LDVMGPQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILKTELAQYGQVFVTTDDGSYGIKGNVSVVINDL-DSQFDAVYSCGAPGMMKYINQTFDDHPRAYLSLESRMACGMGACYACVLKVPESETVSQRV---CEDGPVFRTGTVVL 4YRY Chain:C ((8-247)) -------EIVKKVKIAEDVFDFWIHSPSVSKEARPGQFVVIRLHEKGE--RIPLTVADTKPEEGLFRMVVKVVGKTTHELSLKKEGDTILDVVGPLGNP---SEIENYGNVLLVGGGVGIATLYPIAKALKEAGNNITTVLGARTKDYLIMVDEFKEISDVLLVTDDGSAGMKGVVTD-----RERKFDICWAVGPTIMMKFCT----FGVPIWVSLNPIMVDGTGMCGACRV------TVSGQIKFACVDGPEFR------

General information: TITO was launched using: RESULT: Template: 4YRY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1127 -36529 -32.41 -163.81 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -32.41 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_4YRY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YRY-query.scw PDB file : Tito_Scwrl_4YRY.pdb: