Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIHYNTNQTTLPLEISSFLPQDH--LVFTIEKVVNTDEKEVNHLIKYAMFDKEQKRGYKQSARNLANWHYNDKEDSYTHPDGWYYRFHHTKYQKTQTDFQQEIKVYYADEPESAPQKGLYMNERYQNLKAKEAFICPKTDFRSTQD 4RFU Chain:A ((7-123)) -----APIMTQGSLYNDSLSTNDFKSILLGSTPLDIAPDGAVFQLDRPLSIDYSLGTG---DVDRAVYWHLKKFAGNAGTPAGWFRWGIWDNFNKTFTDGVA----YYSDEQP------------------RQILLPVGTVCTRVDS

General information: TITO was launched using: RESULT: Template: 4RFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 544 6277 11.54 54.58 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 11.54 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.182

(partial model without unconserved sides chains): PDB file : Tito_4RFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RFU-query.scw PDB file : Tito_Scwrl_4RFU.pdb: