Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYGRGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRKITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANHVVVFGG---KEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVVETVVGGV------PVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEILWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKATVERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY 1UM0 Chain:C ((8-359)) LRLTTFGESHGDVIGGVLDGMPSGIKIDYALLENEMKRRQGGRNVFITPRKEDDKVEITSGVFEDFSTGTPIGFLI----HNQRARSKDYDNIKNLF---------RPSHADFTYFHKYGIRDFRGG-GRSSARESAIRVAAGAFAKMLLREIGIVCESGIIEIGGIKAKNYDF----------NHALKSEIFALDEEQEEAQKTAIQNAIKNHDSIGGVALIRARSIKTNQKLPIGLGQGLYA--KLDAKIAEAMMGLNGVKAVEIGKGVESSLLKGSEYND---LMDQKGFL--SNRSGGVLGGMSNGEEIIVRVHFKPTPSIFQPQRTIDINGNECECLLKGRHDPCIAIRGSVVCESLLALVLADMVLLNLTS-KIEYLK-------------

General information: TITO was launched using: RESULT: Template: 1UM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1822 -51312 -28.16 -149.60 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -28.16 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_1UM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UM0-query.scw PDB file : Tito_Scwrl_1UM0.pdb: