Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKTENKVTGVV-TYGEWTT-------GNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRLSTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK 4MT5 Chain:A ((83-182)) --------AEQKTVTETIHYVDENN-QTVQPDSTTAVTFKRGYTTDNVTGKVVSYDPWTVDGNQADSKTFAAVPSPAVEGYT-PNHQQINEFTVTPDSKDIVKTVVYVGD----------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4MT5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 296 12265 41.44 133.32 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 41.44 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_4MT5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MT5-query.scw PDB file : Tito_Scwrl_4MT5.pdb: