Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGE----YRDKNYLEWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQDFSLARRH--SQPSLV---RQGNQPTTPFQ-----KENPGEFVKYSQKLTQSHYILAEE------VHSIPTKNEEVSA-PAPKKNNYDFLKKSQI---------YNKKSKQTEQERRVAQELNLTRMTE----------------------------- 3WUW Chain:G ((16-301)) KPFLSAWPSAVVPRGGHVTLRCHYRHRFNNFMLYKEDRIHIPIFH-GRIFQESFNMSPVTTAHAGNYTCRGSHPHSPTGWSAPSNPVVIMVTGNHRKPSLLAHPGP----LVKSGERVILQCWSDIMF--EHFFLHKEGISKDPSRLVGQIHDGVSKANFSIGPMMLALAGTYRCYGSV-THTPYQLSAPSDPLDIVVTGPYEKPSLSAQPGPKVQAGESVTLSCSSRSSYDMYHLSREGGAHERRLPAVRKVNRTFQADFPLGGTYRCFGSFRHSPYEWSDPSDPLLV

General information: TITO was launched using: RESULT: Template: 3WUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1006 82953 82.46 421.08 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain G : 0.60 3D Compatibility (PKB) : 82.46 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.158

(partial model without unconserved sides chains): PDB file : Tito_3WUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WUW-query.scw PDB file : Tito_Scwrl_3WUW.pdb: