Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYQEETMKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTMFVTGQRSEERRVSI 4ZRB Chain:G ((4-130)) -------MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTMFVTGQRS-------

General information: TITO was launched using: RESULT: Template: 4ZRB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 630 -87265 -138.52 -687.13 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain G : 0.96 3D Compatibility (PKB) : -138.52 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_4ZRB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRB-query.scw PDB file : Tito_Scwrl_4ZRB.pdb: