Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEELIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTIRGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF 4UOH Chain:B ((4-133)) -ERTFIAVKPDGVQRGLIGEIIKRFEAKGFKLAGMKY-IQASEDLLKQHYIDLADKPFYPGLCKYMSSGPVVAMCWEGTGVVKTARVMMGETRPADSKPGTIRGDFCIEVG-----RNIIHGSDSVESANKEIALWF

General information: TITO was launched using: RESULT: Template: 4UOH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 616 -75260 -122.17 -578.92 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -122.17 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_4UOH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UOH-query.scw PDB file : Tito_Scwrl_4UOH.pdb: