Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKRTWRNSFVTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELG-AGLVVMEMVSDKGIQYNNEKTLHMLHIDEGENP--------VSIQLFGSDEDSLAR-AAEFIQENTKTDIVDINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVL--DIPLTVKMRTGWADPSLAVENALAAEAAGVSALAMHGRTREQMYTG-HADLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA 4YCO Chain:C ((8-245)) ---------------------------RVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVD-QLLPVKVFHRI-CPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWG--VDLNCGAPS----GSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLN-IPVIANGEIWDWQSAQQCMAISGCDAVMIGRGALNIPNLSRVVKY-------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4YCO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1298 12797 9.86 57.90 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : 9.86 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_4YCO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YCO-query.scw PDB file : Tito_Scwrl_4YCO.pdb: