Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------MAGILVYGGVQLIGLTANH--------------EMRIFILIILTSLVFMSMVTTLATWNSR---IGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKSFS-- 5ICA Chain:B ((7-152)) TLENYIHAGAYRDAIVLALQLNHPGRLLNLFTNVVTTRNPDPDSLTGLKAVDDVLAKLSDEQIFQLLLRLRDWNTNARTAPVAQRVLWALFKSHPANKLSSLSSLNEVLDAIKVYTERHYKRIEELVDESYLVEYTLR

General information: TITO was launched using: RESULT: Template: 5ICA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 297 -11873 -39.97 -114.16 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -39.97 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.172

(partial model without unconserved sides chains): PDB file : Tito_5ICA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ICA-query.scw PDB file : Tito_Scwrl_5ICA.pdb: