Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDTPLI-IGSRSFQSRLLVGTGKYKDLNETDLAIQASGAEIVTVAIRRVNIGQNPDQPNLLSVIPPEKYTILPNTAGCFDADSAVRTCMLARALLDGHNLVKLEVLGDEKTLYPNVTETLKAARTLIDDGFEIMVYTSDDPIIAQELESMGCVAIMPLGSLIGSGLGILNPHTISIIKENAKVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNPILMASAMKKAVEAGREAFLAGRMPRKRMANASSPETGYFFK 4N6F Chain:B ((12-250)) ---EPWLKIGAREFRSRILVGIEQYDSVPLVRDVLNAAGADVFITTVDPDNRRSSLLLMDLADELPLDDFTWIGTTSFARTKESALRSARILRDSL-GIEILKLDVRGDDNT--PDNAGTVEAARELRAEGMELLPFILPDLATARALEEAGCAALRVMASPVASGRGIANPAAIRELIEQIGIPVVVEGGIGSARHVAEAMELGASATLVNTALVRAESPLLMAAAMRQAALAGLLSYESGPMP-----------------

General information: TITO was launched using: RESULT: Template: 4N6F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1351 -31093 -23.01 -130.64 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -23.01 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_4N6F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N6F-query.scw PDB file : Tito_Scwrl_4N6F.pdb: