Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MWIAISALNLAFAVMLGAFGAHGLKAHASPEQLAWWQTATDYFFYHALG----LLALGIL----SKVLPHFPIKLSFLLIQIGILFFCGSLYIMALGLPRILGAITPIGGALMIAGW-----LILAWNAFKYAK 4MTM Chain:A ((28-164)) IPTATSTTAGITKVLNVLNSNDVGSALSAAQGKVLNDKFNFQNSKNQSGYVRLGDSGLIIQWGVFTSTKTQSNLIFPLAFPNALLSITGNLNSNTPDVIGIDFDLSTATKTSIKTGAAQVGASWLSGKKISWIAIGY--

General information: TITO was launched using: RESULT: Template: 4MTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 333 -32982 -99.05 -277.16 target 2D structure prediction score : 0.14 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -99.05 2D Compatibility (Sec. Struct. Predict.) : 0.14 1D Compatibility (Hydrophobicity) : 0.62 QMean score : -0.039

(partial model without unconserved sides chains): PDB file : Tito_4MTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MTM-query.scw PDB file : Tito_Scwrl_4MTM.pdb: