Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMLAQLQNRFDQWVEQIVQYLDRLSVRERIMVVFTTIFVVVVIVGYSLWKMHSLAEQQQKRLNDLKDLMVWMQSNAVTMKPANELELDKSGKIQRVAQQQGLTVSSQQNGEQLQIVVTHQNYAILANFLIQLAQMGLSIQKMEMVSSEGQIKLTATV 4UZZ Chain:A ((233-340)) ----AASPKQIQMWINNVAEIRKTKQPHS---------------VSYTK-PMPEIDELMQEWPQEIEEILQHLK------IPSEELDFNLSDFCKLACAILDIPVHDQPN-ESNVIESLHVLFTLYSEFKSNQHF-----------------------

General information: TITO was launched using: RESULT: Template: 4UZZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 233 -11362 -48.76 -105.20 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -48.76 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.322

(partial model without unconserved sides chains): PDB file : Tito_4UZZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UZZ-query.scw PDB file : Tito_Scwrl_4UZZ.pdb: