Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGQQRNILATLGIDVWIPRTQVCQKNNAHTLWRDQVVEPHESITVPTIDVPAFEQKNTQPQVFEIPKVVEEPPIVVAEVSQPEILVEKPKVIEQETITPFELQAYCLEKCVIFV---DVTALETEEKQLWANIQ-KAKVGRYSELRWPFPLAAYQDQ---------------RGVGSYIQ-GFLDAVAAEKKILCLGKCAYIQHANIIHLASLKEMLDKPLLKKRLWQLMQD-NNE 1VR8 Chain:A ((5-141)) ------------------------------------------------------------------------------------------KIHHHHHHPP---EAYSLDTAIFVLETRDYRLSDVKEIDSYGDVEMKGKVAVFETEYGPVFLYVYKGEEAKKIWKKLNGRVSIRSVLDLPNMGKFSTVSNGKKIVAWWR------KNWLFIVEGKNGVEE--FVKHVYRVYEEMKQ-

General information: TITO was launched using: RESULT: Template: 1VR8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 476 -6296 -13.23 -55.71 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -13.23 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.206

(partial model without unconserved sides chains): PDB file : Tito_1VR8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VR8-query.scw PDB file : Tito_Scwrl_1VR8.pdb: