Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------MKKQLPKISTTSKALAKSLLLAQGVLDQAKKYSTLPFTQ--THIILPRIDEKYYSWTHYGIF--FPLLPEPHRYLNIMILIGTPGALAFDHDDI-----------------------------ITGN---PRKTATFFSSTAALEQALLKAYIIPEDTKINKDGTLIELGQEI---------SIQGKFPHIHINGHYD----------GFDFDFDID----------------ITSHVSWFIKTPIYDHFSLLAKFKGFLNYQAKHIE----TQGLCTYEYARAVGPHSITNKL--IPDAYKLPLDFFT-------------------------------YQIINLNEATQLLLTKADIAGQTA-----AYTLHIRHLDQPAEIYTDVSFDIISHQVDDFVSPSGQKMRLPKYFSWIARNDAKQIILNIQAEIDCPFRYGHGRGYAS-------SYIFTGHYFGNEVQG-RGYIEYVDIENPQAFEDE-- 5FQL Chain:A ((9-525)) TDALNVLLIIVDDLRPSLGCYGDKLVRSPNIDQLASHSLLFQNAFAQQAVAPSRVSFLTGRRPDTTRLYDFNSYWRVHAGNFSTIPQYFKENGYVTMSVGKVFHPGISSNHTDDSPYSWSFPPYHPSSEKYENTKTCRGPDGELHANLLCPVDVLDVPEGTLPDKQSTEQAIQLLEKMKTSASPFFLAVGYHKPHIPFRYPKEFQKLYPLENITLAPDPEVPDGLPPVAYNPWMDIRQREDVQALNISVPYGPIPVDFQRKIRQSYFASVSYLDTQVGRLLSALDDLQLANSTIIAFTSDHGWALGEHGEWAKYSNFDVATHVPLIFYVPGRTASLPEAGEKLFPYLDPFDSASQLMEPGRQSMDLVELVSLFPTLAGLAGLQVPPRCPVPSFHVELCREGKNL--LKHFRPGNPRELIAYSQYPRPSDIPQWNSDKPSLKDIKIMGYSIRTID--YRYTVWVGFNPDEFLANFSDIHAGELYFVDSDPLQDHNMYNDSQGGDLFQLLMP

General information: TITO was launched using: RESULT: Template: 5FQL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2092 32445 15.51 89.38 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 15.51 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.099

(partial model without unconserved sides chains): PDB file : Tito_5FQL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FQL-query.scw PDB file : Tito_Scwrl_5FQL.pdb: