Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------MKKPVPKIYRTTNW---SSYNQALIKRGN---ISIWFDPKTQWYAQPQGKHGRNQTYSDAAIQCCLMIKSLFRLSLRMVTGF---------------------AQSLIKLCGL-NWTAPDYSTL-----------CRRQKRIDIA--ISYQQSRDGLHLLVDSTGLKF-----LGEGEWKR-KKHQPAYRRQWRKLHIGIDAK-----TLQIRAVQLTTNNVS-DSQVLGDLLDQIPLDERIDSVYTEGAYDTKHCRQVILDRDAHAVI--PPRKNA-KPWKDKKLRSLERNELLKTVKRLGRSLWKKWSGYHRRSLVETKMHCIK--------------------- 5T6O Chain:A ((1-389)) SAFEVGRNVAVTEGAVVFENEYFQLLQYKPLTDKVHARPLLMVPPCINKYYILDLQPESSLVRHVVEQGHTVFLVSWRNPDASMAGST-----WDDYIEHAAIRAIEVARDISGQDKINVLGFAVGGTIVSTALAVLAARGEHPAASVTLLTTLLDFADTGILDVFVDEGHVQGAPCALLRGLELANTFSFLRPNDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLPGPWYCWYLRHTYLQNELKVPGKLTVCGVPVDLASIDVPTYIYGSREDHIV---PWTAAYASTALLANKLRFVLGASGHIAGVINPPAKNKRSHWTNDALPESPQQWLAGAIEHHG-SWWPDWTAWLAGQAGAKRAAPANYGNARYRAIEPAPGRYVKAKA

General information: TITO was launched using: RESULT: Template: 5T6O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1321 -13924 -10.54 -53.35 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -10.54 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.038

(partial model without unconserved sides chains): PDB file : Tito_5T6O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T6O-query.scw PDB file : Tito_Scwrl_5T6O.pdb: