Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------MKNIRIGSYHWMQTTNGQLSFKEKIKLIQKIMVPSILSSI-----KINYYRFQTGNDFDIGQI-------------------------VIPDTQMIKIALEELESK---------------ASISIYNHS--W-------------RTYFWGAALGHLQNQQFDPESLLLASLFH---------DIGLTEQHM------HSKGCQCFTYESAKQ------FEQKAKEYNFDDKKSEVIKDAICLHMNGYIEDSDPPEVVLLQQGASCDVI---SDNQYKLPLSFRNKILEKYPRN-----QFNKEFIKLINLERKN-VPNSRTG-LLYDLGLPLMIKS--NLYNENLI------------------- 2V4J Chain:B ((2-381)) AFISSGYNPEKPMANRITDIGPRKFDEFFPPVIAKNFGSWLYHEILEPGVLMHVAESGDKVYTVRVGAARLMSITHIREMCDIADKYCGGHLRFTTRNNVEFMVADEASLKALKEDLASRKFDGGSLKFPIGGTGAGVSNIVHTQGWVHCHTPATDASGPVKAIMDEVFEDFQSMRLPAPVRISLACCINMCGAVHCSDIGVVGIHRKPPMIDHEWTDQLCEIPLAVASCPTAAVRPTKLEIGDKKVNTIAIKNERCMYC-GNCYTMCPALPISDGEGDGVVIMVGGKVSNRISMPKFSKVVVAYIPNEPPRWPSLTKTIKHIIEVYSANAYKYERLGEWAERIGWERFFSLTGLEFSHHLIDDFRDPAYYTWRQSTQFKF

General information: TITO was launched using: RESULT: Template: 2V4J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1009 4963 4.92 19.54 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 4.92 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.165

(partial model without unconserved sides chains): PDB file : Tito_2V4J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V4J-query.scw PDB file : Tito_Scwrl_2V4J.pdb: