Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGSTITPWVVGNWKMNPMRANANQLIEEFKQLLQQNQIADENCHVGVAPVSIAL--TTVQAQLQDAARTVYTVAQDVSRVAGTGAYTGEVSAELLKDSQINFVLVGHSERRDIFGDNVEILKAKLQNALNAGMTVIYCVGESLEQREQGQAEQVVLQQICDIAPVVTAEQWQNQVVIAYEPIWAIGTGKTASPQDAQAMHAKIREGLCQLTPA--GSNIAILYGGSVKAENAVELAACPDINGALVGGASLNAASFYQIVQAFAQSK 2I9E Chain:D ((7-245)) ---------VGGNWKMNGDKKQINEIIG-FLKSGPLNQ--DTEVVVGVPAIYLELVRTCVPASIGVAAQNCYKVPK--------GAFTGEISPAMIKDVGADWVILGHSERRQIFGESDELIAEKVCHALESGLKVIACIGETLEEREAGKTEEVVFRQTKAIAAKVN--DWSN-VVIAYEPVWAIGTGKTATPQQAQDVHKALRQWICENIDAKVGNSIRIQYGGSVTAANCKELASQPDIDGFLVGGASLK-PEFVDIINA-----

General information: TITO was launched using: RESULT: Template: 2I9E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1256 -14993 -11.94 -63.80 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.81 3D Compatibility (PKB) : -11.94 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_2I9E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I9E-query.scw PDB file : Tito_Scwrl_2I9E.pdb: