Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFTRSQIFLACALASSLLFSACSKENKPQKEDALTATAPATGEASTIIERNAKQRLIETLQKQFKNANINVKILDIKPTEVPNLYWVNLEGMTSVYTTSDGKYLIQGDVIRLGGKELHNIGDSLQASENKKHLAALKNEDLIAYPAKGGKAKHVIYVFTDVSCPYCHKLHEHLAEINEKSIEVRYIAWPR---GEQFMPTMEAVWCSADRKAAFNQAVQGINIPPAQCKNPVREQYQLGLNMGVNGTPAIYNVDGEYLGGYLTPDELIKRLDK-------- 1EEJ Chain:A ((1-216)) ------------------------------DDAAIQQTLAKMG----------------------------IKSSDIQPAPVAGMKTVLTNS-GVLYITDDGKHIIQGPMYDVSGTAPVN-------VTNKMLLKQLNALEKEMIVYKAPQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIWCAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQPPKEMKEFLDEHQKMTSGK

General information: TITO was launched using: RESULT: Template: 1EEJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 970 -68741 -70.87 -335.32 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -70.87 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.671

(partial model without unconserved sides chains): PDB file : Tito_1EEJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EEJ-query.scw PDB file : Tito_Scwrl_1EEJ.pdb: