Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MLKINDFPHDNNFWKVEWIGGVYNDIDGEPRIRIYLAQIKSNIERQKTLYNS-SLEVRNQQNISSVHDVKIGLI----------QFIPIGSVWKNGILDQSFTAQYEQFNFNSDTPSEVCFQSQYGGIAETGLNNTTEWNNHLNDFHYRIVNSEARALGNYINVFYDYNGLSRIYIPSIVILQ-------ACYITSPKSAR------HIIFGQINKLLDPETSGFIDKETFRIHLHRDYKDIEAPMIANLATNFNAKMGLRTLRQSLVKESNITSISTPKRLKISFPFSNDFSIKVKGKYIMENNKIYGFFVTQII--------EFSTKFDFTKLVIFRKNSNQKGKEKAENLQNAYE----GCNAVRPSHSESLDDKDESQPLIFTHDQVSNQNLPQSIDFPLCSNISNLEVLKE-----DKDIQYYQNASYTNLNLRPSNTTSTGPAISSNNGVSELQIEPSLPVDLEYFFDVLEILNKLGYQFKS-------IAVNNSRDHPRGIINQFNRFIKNLHKWHLQDDDQTPRPFIVAEMIYKGAFYYLLDIAPRKGAALS----AQLIRAYTGEQITPTAFAILLNDVARTS--PKGWSVL------------------DHAKYSQKWQHFRIEHNRNNKPELVAKNIIKKLG-------------- 4HG6 Chain:B ((36-702)) PWIIPLRPLAETAQVGPLFRLQGQ-------QARAAFRLFLPTEAVGGTLTLAQRSSIDILPESSQIIVRMNDQEIGRFTPRQFGALGAVTMPLGEAVRAGDNLVTIEAQH-RHRIYCGADAEFDLWTEVD-LSQSGVALPAAAIGTEPTSFIAALTAQAESGRPVEIRTPTPPDEATLRTLAQALGRPLPDEALPLALSKPWSAETGPTYARITLLPSDADRVSIRRGGDGAVVLVLEHPPE-GSPNASLVADLLGATPTLPPPTLPQIPPGRVVTLADMGVDTILTDNRYFNRDIDFQLPDDWLLLASQKAQIGIDYGFAGGLPEGALLLVKVNGTTVRMLPLDRDAAPVKPRLDIRFPARLLHPGPNRLSFESVIPGNPPDQPCPASAGDLMQVLSST--DLEVPPSPRMQMADMARDLAQVTPASVHPATPDGLARTLPFMAAFREVPDAAPVDLTVAGLHDIATVPLNEEGLTPRLLALTLLPSTGPPANALAPLGAAPGEGVMPPLVESNWSDRAQTFVQATLQPVIQT-----VRRMLRPGDGNLAEWLATRKGTAMLLAPEPGKLWVILGPEAEPARVAEALAMAPRSPGGPRGQVAVLGSDGRWSSWSKPGLLPELREPVSLDNVRSVVGNVASARPPLLLGGMLGLAWISAAIAVGFVLRTR

General information: TITO was launched using: RESULT: Template: 4HG6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2915 89832 30.82 159.28 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : 30.82 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.111

(partial model without unconserved sides chains): PDB file : Tito_4HG6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HG6-query.scw PDB file : Tito_Scwrl_4HG6.pdb: