Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPRPITAVIHRQALQNNLAVVRKAMPNSKVFAVVKANAYGHGIERVYEAFKAADGFALLDLEEAKRIRALGWTGPILLLEGVFSPQDLFDCVQYQLSFTIHSEAQIEWVEQHPYPAQFDVFLKMNSGMSRLGFKPQHYVQAWERLNNLANVAKITHMM-HFSDADGDRFGQQGIDYQITAFEEIVKDLPGERSVSNSAAILRYQDQLKSDYVRSGIMLYGSSPDYPTHSIADWGLQPTMSLRSEIISVQHLEPNESVGYGSNFVAEQPMTIGIVACGYADGYQRISPTGTPVLVDSVRTRTVGRVSMDMLAVDLTGIESAKVGSEVVLWGQSSTGVVLPIDDVAVSSGTVGYELMCAVTARVQFINQV 4XBJ Chain:C ((5-353)) -----TVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEA-LDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCG--ATEKQLAIFNTFCEGKPGQRSIAASGGILLWP-QSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVS-RDTRLGVVAMGFGDGYPRAAPSGTPVLVN-REVPIVGRVAMDMICVDLGPQAQDKAGDPVILWG-G-----LPVERI--MTKVSAYELITRLTSRV------

General information: TITO was launched using: RESULT: Template: 4XBJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1979 -147187 -74.37 -430.37 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -74.37 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_4XBJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XBJ-query.scw PDB file : Tito_Scwrl_4XBJ.pdb: