Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAAPRIGILGAGGRMGRTLIQA-VQQAGYQLAAAVERPESSLVGTDAGELAGIGSVGVKVSGSLADVLKDCDVIIDFTAPAATAQHLKLCREAGVAMVIGTTGMSDEQKAELDEAATHIPVVYAANYSVGVNVSIKLLELAAKVFGDTVDIEVIEAHHRHKVDAPSGTALMMGEAIADTLGRNLKEVAVYGREGHTGPRDRQTIGFETIRGGDIVGEHTVMFIGEGERVEVTHKATNRMNFAAGAVRAAAWVVGREARKYDMKDVLGLNDVQV 1ARZ Chain:D ((7-273)) -----RVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSLLGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIGTTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHHRHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVGEHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL--

General information: TITO was launched using: RESULT: Template: 1ARZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1434 -39098 -27.26 -146.98 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain D : 0.88 3D Compatibility (PKB) : -27.26 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_1ARZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ARZ-query.scw PDB file : Tito_Scwrl_1ARZ.pdb: