Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLVVFSGAGMSAESGISTFRDSNGLWENYDIQQVATPEAWERNPALVQRFYNERRKNILEAQPNEAHQYIAKLQDHYDVQ-VITQNIDDPHERAGSQNVLHLHGNIRLAKSSGPDAQYTTQFYEVNGWKLDLEQDFCPNGYPLRPHVVWFGEAVP--AYEEAIRLVQSADIFIVIGSTLSVYPVAALVHEIPHYSKAYYIAPQADHSRVPPQYKLLNMTATEGMHELFNQLTS 1M2N Chain:B ((15-196)) ---LVALTGAGVSAESGIPTFRGKDGLWNRYRPEELANPQAFAKDPEKVWKWYAWRMEKVFNAQPNKAHQAFAELERLGVLKCLITQNVDGLHERAGSRNVIHLHGSLRVVRCTSCNNSFEVE----SAPKIPPLPKCDKCGSLLRPGVVWAGEMLPPDVLDAAMREVERADVIIVAGTSAVVQPAASL----------------------------------------------

General information: TITO was launched using: RESULT: Template: 1M2N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 845 -24402 -28.88 -136.32 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -28.88 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_1M2N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M2N-query.scw PDB file : Tito_Scwrl_1M2N.pdb: