Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKSFFPSPRYFFISVVIWLALNMVLWYTGGDHWGQYLGFPQGYADAELPIGVSRFWSPAFLWFYLWFLVSTALFAGFWKIISNNPWQRWSIWGAAFILFNIWFAVQVNVAINAWYVPFWDLIQKMLSSGGGDISTLYSETLVFLYIAMVYVTLGVITAFF----TSHYVFRWRTAMNEYYTEH---WEKLRHIEGASQRVQEDTMRFATIMEDLGVELVKSVITLIAFLPILFHLSKYVLVLPLVGELNHSLVWAAIVWSIFGTVLLMVVGIKLPGLQFNNQKVEAAYRKELVYGEDHAE--RA--QPVTLKELFANVRKNYFRLYFHY----AYFNMTATWYGQLDILYNLVVLFPSIAAGKLTLGLMQQIMNVFGRVRDSFQYLISSWKTIIELLSIYKRLKAFESILHK 3WMG Chain:A ((33-328)) --------------------------------------------------------------------------------------------------FIASILEGATLPAFAIVFGRMFQVFTKSKS---QIEGETWKYSVGFVGIGVFEFIVAGSRTALFGIASERLARDLRVAAFSNLVEQDVTYFDRRKAGELGGKLNNDVQVIQYSFSK-LGAVLFNLAQCVVGIIVAFIFAPALT-------------GVLIALSPLVVLAVVVQMIEMSGNTKRSSEAYASAGSVAAEVFSNIRTTKAFEAERYETQRYGSKLDPLYRLGRRRYISDGLFFGLSMLVIFCVYALALWWGGQLIARGSLNLGNLLTAFFSAILGFMGVGQAAQVWPDVTRGLGAGGELFAMIDRV--

General information: TITO was launched using: RESULT: Template: 3WMG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 828 -99848 -120.59 -355.33 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -120.59 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_3WMG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WMG-query.scw PDB file : Tito_Scwrl_3WMG.pdb: