Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGQQLVFKSKNA-------LTDLTAIPDFVSYKS-----RV--------ESDGGVIYDQQA------VLDVLQFIFQNSIPETDILSATSAAWGVKFDPVT------KVITKLYNLFNPAGD------IIIQNGTMNAIHTTEVDG-----KPSLYAGGTSTLYGYSSGKFAIANPI---------AHTIH---HVPARSGYGASALLFP----LQTLFNKE---GFDASTDPAKVSTD----YVAVDQ------RLTRNA---TTNNDPNTWNEAHRFWGASVGVDGNLNGSRIGYASNALTTGTFIYKDGAQSNSNSTV----PNIKPTQQADQRLYLMSNFTVAGVRGNYYLGYIFENWLLNNGTDSIAKAL---SLRAKTKYR 1SQ9 Chain:A ((3-395)) ---KVFIATANAGKAHDADIFSVSACNSFTVSCSGDGYLKVWDNKLLDNENPKDKSYSHFVHKSGLHHVDVLQAIERDAF-ELCLVATTSFSGDLLFYRITREDETKKVIFEKLDLLDSDMKKHSFWALKWGASNSHRLVATDVKGTTYIWKFHPFADESNSLTLNWSPTLELQGTVESPMTPSQFATSVDISERGLIATGFNNGTVQISELSTLRPLYNFESQHNNSNSIRSVKFSPQGSLLAIAHDSNSFGCITLYETEFGERIGSLSVPGEFAHSSWVMSLSF--NDSGETLCSAGW---DGKLRFWDVKTKERITTLNMHCDDIEIEEDILAVDEHGDSLAEPGVFDVKFLKKGWRSGMGADLNESLCCVCLDRSIRWFREAG

General information: TITO was launched using: RESULT: Template: 1SQ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1623 59761 36.82 201.90 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 36.82 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.104

(partial model without unconserved sides chains): PDB file : Tito_1SQ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SQ9-query.scw PDB file : Tito_Scwrl_1SQ9.pdb: