Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEFLKAYVQTFKDIVSNSSAFTTLFLSVILYSFFYPTAYKAERAESIPIVIVDEEQSLLTSQVIGQTANSPHVKIVDVTANFLEAEQMVREQKADGILLLPSNLTQSLRRGETGGIGLYLSTTNFLKTKEIGLGLATSIEATLKEYIERFGQRTHFQPALSIHQMPLFNTLSGYGSYIFPAVASLIIHQTIVLGLAMLVASYREQHEKITPIRFAGIFASIFTIGCLGSFYLFGFTLWFNDYPHGGNFVGLLVAVPIFISCVIGLGMLIGSLLDILERAGHIIVFSSVPLFLLTGAAWPHQAMPEWLQWFAWCLPSTHAVQMFVQLNQMGVPLNVVAPKLIFLATIGVIFLITAYSRLKVSK 5DO7 Chain:C ((365-414)) FSKLGVLLRRVTRNLVRNKLAVITRLLQNLIMGLFLLFFVL----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 18 -4791 -266.17 -116.85 target 2D structure prediction score : 0.83 Monomeric hydrophicity matching model chain C : 0.44 3D Compatibility (PKB) : -266.17 2D Compatibility (Sec. Struct. Predict.) : 0.83 1D Compatibility (Hydrophobicity) : 0.44 QMean score : 0.129

(partial model without unconserved sides chains): PDB file : Tito_5DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DO7-query.scw PDB file : Tito_Scwrl_5DO7.pdb: