Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIISKDIKMLALVTGASAGFGYSISKKLIESGYNVIGCGRRAEKLEELQKQLGENFYPLVFDMTDTAENINKLFKELPNEFQIDQIDLLVNNAGLALGLEPADKADLDDWYTMIDTNVKGFVTVTRLILPSMVKKKSGLIINMGSIAGTYPYPGGNVYGATKAFVEQFSLNLRADLAGTGVRVTNIEPGLCGGTEFSLVRFKGDQEKANSLYDKKNPILPEDIANTVAWIASQPPHININRIEMMPTTQTFNPLKVVEVE 3ASV Chain:E ((1-245)) --------MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDELGDNLYIAQLDVRNRAA-IEEMLASLPAEWC--NIDILVNNAGLALGMEPAHKASVEDWETMIDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLNLRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWWVSTLPAHVNINTLEMMPVTQSYAGLNV----

General information: TITO was launched using: RESULT: Template: 3ASV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1291 -128571 -99.59 -524.78 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain E : 0.88 3D Compatibility (PKB) : -99.59 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.601

(partial model without unconserved sides chains): PDB file : Tito_3ASV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ASV-query.scw PDB file : Tito_Scwrl_3ASV.pdb: