Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIEKFSVEKVLSVHRWTPTLFSFTMTRPSHFKFTAGQFARIGLKVGDELIVRAYSVVSSPFDETLEFFSIVVPDGAFTSNLQHLKVGDELYLEKIPYGYLTLARYQQPLPHDLWLLATGTGLAPFLSMLQDFETWSNYQKINLVYSVRTAAELAYVDRIQEIAE-TFGEGHLGFKFIPIITR--DPSAPLHERLPVLIENGELEKVAGLELSPASSHVMLCGNPQMVDDTKEALKRCGLTMNR-RGEGNIAVENYW 5UFA Chain:B ((16-265)) ----KFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSRLGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATGFLLPERF--PDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDRLAALSEH-------SFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPESTRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMEN--

General information: TITO was launched using: RESULT: Template: 5UFA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1277 -118132 -92.51 -496.35 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -92.51 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.578

(partial model without unconserved sides chains): PDB file : Tito_5UFA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UFA-query.scw PDB file : Tito_Scwrl_5UFA.pdb: