Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDETKMSYQSIFRPDAFANKVIIVTGGGSGIGRCTAHELAALAAQVVITGRKIEKLEKVSQEIIED-----GGLVHFIVCDNREEEQVKNMIAEVIEKFGKLDGLVNNAGGQFPSALENISANGFDAVVRNNLHATFYLMREAYNQWMAKHGGSIVNMTADMWGGMPGMGHSGAARSGVDNLTKTASVEWGKSGVRVNAVAPGWIVS-SGMDNYSGDFAKVIIPSLAGNVPLKRMGTESEVSSAICYLLSDAAAFVSGVTLRIDGAASQGTRMYPLAEATNSQSYNGFHRAFIPEIFKKDKTEKVEQSNNSGSE 1YXM Chain:B ((8-279)) --------RSYLAPGLLQGQVAIVTGGATGIGKAIVKELLELGSNVVIASRKLERLKSAADELQANLPPTKQARVIPIQCNIRNEEEVNNLVKSTLDTFGKINFLVNNGGGQFLSPAEHISSKGWHAVLETNLTGTFYMCKAVYSSWMKEHGGSIVNIIVPTKAGFPLAVHSGAARAGVYNLTKSLALEWACSGIRINCVAPGVIYSQTA---------QSFFEGSFQKIPAKRIGVPEEVSSVVCFLLSPAASFITGQSVDVDGGRSLYTHSYEVPDHDN---------------------------------

General information: TITO was launched using: RESULT: Template: 1YXM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1417 -65957 -46.55 -255.65 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -46.55 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.441

(partial model without unconserved sides chains): PDB file : Tito_1YXM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YXM-query.scw PDB file : Tito_Scwrl_1YXM.pdb: