Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTY--ALSPILRKL--YKDDDEYIEALNNHFQYYNTQPWLAAIILGACV--------------AMEEKQGLEAKDAINDFKVGTMGPIA------GIGDSLLMTMIPTIMGSIAAYMALENNPVGIF--LWFILIMVIFFFRMRGFEFGYKQGMKIVTEYGEKINYLTEAASVLGLTV--------VGSLIASVISVSTPLKFTFGE----VSMKIQPMLDKILPTLIP---VIITAAAYYLLSKKKIS------MTILILLVIVF------------AMVMAAFGILA--------------- 4MFL Chain:A ((23-345)) ---MDPETVRIALGLEERTAAWLTELDELG-PPAEPVRLPRGEEARDLLRRLEVPELDAEEIVAAAPDPDRDPALWWLLERTHHAIVRHMGDHRAKPRGGPPLPYEGGAAARYFHVYVFLATVPAVRRFHAERGIPDEVGWETL-TQLGELVAIHRRKYGQGGMNMQWWTTYHLRGILYRLGRLQFSLATG--------------KDGTPHLGLAVPEWGGPLLPKAYDESLHRARPFFDRHFPEHGARVAWGSSWMLDPQLEEYLTEDSNIIQLARFWTLTDSAPEPGNADGDSSILEFVFRYNGQPLDELPQRSSLERAVIAHLKAGRHWHMRTGFVKLP

General information: TITO was launched using: RESULT: Template: 4MFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1149 -66661 -58.02 -267.71 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -58.02 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.260

(partial model without unconserved sides chains): PDB file : Tito_4MFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MFL-query.scw PDB file : Tito_Scwrl_4MFL.pdb: